![]() This filter is rated up to a 70 gallon (265 liter) tank, and Fluval filters are known for their quality and reliability.īut, it has a somewhat small biomedia capacity. It also comes with a Fluval C4 hang-on-the-back power filter. The kit comes with a sleek LED light fixture that fits onto the curved lid. The curved glass even makes your fish look bigger. You can see the tank much better from all sorts of different angles, not just straight on. I have two of these and I highly recommend them. If you’ve never seen one, on a bow front tank, the front panel of glass curves out. This 45 gallon bow front would make a gorgeous home for a single common or comet goldfish or a pair of fancies. Fluval C4 Hang-on-the-Back Power Filter.45 gallon (170 liter) bow front aquarium.Last update on / Commissions Earned / Images from Amazon Product Advertising API 4.Fluval Premium Bow Front Aquarium Kit with LED, 45 Gallon Have to buy fluorescent bulb separately.Great home for 1 single-tailed goldfish or 2 fancy goldfish. ![]() Tank is much lighter than glass when empty.Acrylic is clearer than glass and provides seamless viewing.I’d say plan on purchasing a light separately. Second, some users complain that the fixture is underpowered and they weren’t really happy with it. First off, it’s a fluorescent fixture that comes without a bulb, so you have to buy that separately. The light that comes with this tank is somewhat problematic. But, it can make cleaning a bit more difficult and it may be impossible to add large decor pieces. The cutouts are big enough to add gravel, fish, water, etc. The inside of the tank can only be accessed through cutouts. The top of the tank is not completely open like most aquariums. There are three options for the back wall of the aquarium, it comes in cobalt blue, black or clear. This acrylic 40 gallon (151 liter) tank gives you a seamless view of your fish. Fluorescent light fixture (bulb not included).Offers a variety of background color options.Last update on / Commissions Earned / Images from Amazon Product Advertising API 3. Beginners may be uncomfortable picking out their own equipment.Let’s you decide which equipment to use.This may not be the best choice for beginners that aren’t comfortable with picking out their own equipment. This would give a goldfish more room to swim before they had to turn around, which is good. You would need to pick out a lid, light and filter to go along with it.Ī 20 long means that the tank is longer and more shallow than a 20 high. This is just the tank, not a kit of any kind. This glass 20 gallon (75 liter) glass aquarium would work well for a single fancy goldfish. You should plan on providing at least 20 gallons (75 liters) per fish. Plan on having a huge tank if you want to have multiple fish, at least an 80 gallon (303 liter) for a pair of comet goldfish.įancy goldfish don’t get as massive as comets and common goldfish, and aren’t overly vigorous swimmers, but they still need ample room. But if you’re going to keep them in an aquarium, expect that they will need at least 40 gallons (151 liters) per fish. Larger and more active breeds of goldfish include: Since they also reach lengths of 12 inches, these fish need a larger, and preferably longer, tank than fancy goldfish varieties. Breeds like comet or common goldfish are very athletic and swim rapidly all day long. Some varieties of goldfish are much more active than others. So they need a higher water volume to dilute that (more on that later). Not only do they grow really large, goldfish also produce an enormous amount of waste and they’re messy eaters. The cute little goldfish you see in the store will grow to be between 6 and 12 inches long (15-30 centimeters), depending on the breed. Here’s the thing: goldfish need a lot more room than people realize.Īnyone who tells you that goldfish can be kept in a bowl or a small 5 gallon (19 liter) tank does not know what they are talking about. Last update on / Commissions Earned / Images from Amazon Product Advertising API What Tank Size Do You Need for Goldfish? SeaClear 40 gal Acrylic Aquarium Combo Set, 36 by 15 by 16", Clearįluval Premium Bow Front Aquarium Kit w/LED, 45 Gallon (170L)Īqua Culture Aquarium Starter Kit, 55 Gallon Aqua Culture 29 Gallon Aquarium Starter Kit with LED
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In fact, unlike most other fliers and their flying methods, Charmy appears capable of sustaining flight indefinitely on his own without his flying appendages ever tiring out, as he is always seen flying around. Thanks to his wings, Charmy is capable of flight. Powers and abilitiesĪlthough neither clever or strong, Charmy has proven himself an asset to the Chaotix Detective Agency. It appears that Charmy has a fondness for money as well, much like Vector, as they got the same glint in their eyes when they were promised a handsome reward for their services at the beginning of the Neo Metal Sonic incident. If any puzzling cases occur, Charmy will be on the case with his flexible mind. This makes him an essential member of the Chaotix Detective Agency. Charmy is also great at coming up with ideas that that no one would think of. In addition, because his childlike innocence gives him a viewpoint his elder coworkers does not have, he has excellent intuition, and he often points out things that no one else would notice. However, he is the life of the party who enlivens the Chaotix Detective Agency with his cheerfulness and carefree attitude. ĭue to his age, Charmy is not the best of detectives. When he does get provoked however, he often introduces the source of his anger to his stinger and delivers a painful sting to them. Not known for his temperament, Charmy is generally good natured and light-hearted, and does not get angry very often. However, he works hard to help his partners. When the team is receiving their first mission at the beachy Seaside Hill, Charmy is hovering around singing "Sea, sea, sea". In fact, as a child, he loves to play above all else (especially work), and he can always be seen flying around energetically. Ĭharmy is curious, playful, always upbeat, and likes to fool around and crack jokes. Not remarkably clever, Charmy is the type of person who takes action first and asks questions later, and when he realizes the error he has made, it is too late. Good-shaped yet sloppy, Charmy is a hyperactive and scatterbrained funny-kid. His striped torso was completely exposed, and he wore red sneakers with white toes and laces, alongside puffy red armbands up near his shoulders.Ĭharmy's profile, from the Xbox 360 version of Sonic Generations. He also wears white and orange shoes cuffed with black rings and topped with yellow zippers, white gloves with similar cuffs, and a black pilot's helmet with a raspberry and two orange-circulated trim, alongside orange and silver flight goggles with light blue lenses.ĭuring the Chaos Ring incident, Charmy was depicted with black eyes, a slightly longer nose, a yellow stinger, and a more pinkish tint to his skin. He also has exposed peach skin on his muzzle and arms, a tiny black nose, and a silver stinger.įor attire, Charmy wears an orange vest with a bee emblem on the left, a small yellow zipper, and a white collar resembling that of a polo shirt. However, his intuition is nonetheless an invaluable assist to the Chaotix Detective Agency, and he serves as a crucial part of their team.Ĭharmy Bee is an anthropomorphic black-and-yellow striped bee with yellow-and-black striped antennae, translucent sky blue wings on his back, and honey brown eyes. Always flying around energetically and annoying his coworkers, Charmy is hyperactive, childish and loves more to play than work. Regardless, he is an excellent flier and possesses a sharp stinger to boot.Ĭharmy's trademarks are his flight suit and helmet. He is as well generally seen by others as his detective agency's entertaining and incredibly cute mascot. Although he is a certified detective, this frivolous and scatterbrained bee is more akin to a mood maker who helps excite Team Chaotix and make his coworkers look professional. He is an anthropomorphic bee and the youngest member of Team Chaotix, a detective agency he helped co-found. Oh flower, pretty flower, show your face and I'll sting you!Ĭharmy Bee ( チャーミー・ビー, Chāmī Bī ?) is a character that appears in the Sonic the Hedgehog series. ![]() Little did she know that the gunman began his rampage at a house close to where she lives with her mother, Carmen. Jasmine Franco, 22, was in a classroom at Santa Monica College – next to the library – waiting for her English class to start at noon on Thursday. The gunman then abandoned the hijacked vehicle – leaving the driver unhurt – and ran, shooting at police, Lewis said. Franco’s vehicle crashed after the shooting. He was carrying an “AR-15 style rifle,” pistols and more clips for the rifle, Lewis said.Īs the car pulled onto the campus, the man fired into Franco’s red SUV, killing him and critically wounding his daughter, police said. Richard Lewis said.Īs the car headed toward the campus of the community college, where 30,000 students are registered, he opened fire on a passing bus, slightly wounding three people, Lewis said. The gunman then got into the first car and forced the woman to drive off with him, police said.ĭuring their ride, 911 calls poured in, keeping police on the gunman’s path, Santa Monica police Sgt. Authorities said that driver was hospitalized and is in stable condition. The motorist was wounded in the shoulder, she said. That driver, also a woman, hesitated for just a moment, and the gunman “fired three shots directly into her and the car,” Cunningham said. She then saw the gunman force a woman to stop her car at gunpoint.Īnother car approached. She saw the gunman firing at a neighboring house that was on fire. Jerry Cunningham stepped onto her porch when she heard the shots. IReporter: There was a body on the corner Investigators are still trying to determine the motive in the shootings that also left four people wounded. His apparently random gunfire became the nation’s latest disturbing killing spree, ending with his death in the college library and leaving this tourist beachfront city reeling with shock. Because he was wearing a ballistic vest and was heavily armed, “I would say it’s premeditated,” she said. Seabrooks estimated the gunman had about 1,300 rounds of ammunition during the rampage. The assailant dressed in black and carried an assault-style rifle. One of the five people killed was shot outside the library of the school, college Police Chief Albert Vasquez said. The 13-minute shooting spree that followed spanned several parts of Santa Monica. Source: Santa Monica gunman previously hospitalized for mental healthĪuthorities believe the house was set on fire before Sam and Chris Zawahri were shot. The gunman and a family member had been enrolled in Santa Monica College as recently as 2010. It’s also unclear under what circumstances he was released.Īuthorities have found no link to domestic or international terrorism, the official added. It’s not clear whether the state government or his family committed him for treatment or whether he committed himself. A few years ago, he was hospitalized for treatment after allegedly talking about harming someone, according to the official. Police had contact with the Zawahri in 2006, but because he was a juvenile, authorities couldn’t release further information, Santa Monica Police Chief Jacqueline Seabrooks said.Ī law enforcement source with knowledge of the investigation told CNN on Saturday that the gunman had suffered from mental health issues. “We are shocked and deeply saddened by the tragedy that … once again, shatters our nation’s confidence,” Superintendent Sandra Lyon said. The superintendent of the Santa Monica-Malibu school district said Zawahri attended a high school for students behind in academic credits in 2006. Scant details are trickling in about Zawahri, who was killed by police at Santa Monica College on Friday – the day before his 24th birthday. The suspect in the shootings has been identified by Santa Monica police as John Zawahri.Īuthorities say he killed his father, Samir “Sam” Zawahri, and brother, Chris Zawahri, in a Santa Monica house before carjacking a woman and firing at a public bus on Friday. John Zawahri in a 2006 yearbook photo from Santa Monica High School. ![]() Signeasy simplifies the signing process for you so you can just focus on important tasks at hand. With Signeasy, you can electronically sign PDF documents online while working remotely. Want to make your PDF more interactive? Learn how to easily hyperlink your PDF documents. Click the Signature icon in the Markup tools.At the top of the screen, you’ll find the menu bar.Open the PDF document you want to sign in Preview.Follow these simple to learn how to eSign PDF on Mac. If you own a Mac, then you have the privilege to use the built-in Preview app to sign a PDF. When you’re ready, click on the Apply button.Drag, resize and position the signature inside your PDF file.You will be given three options to add signature to PDF- Draw, type, and upload an image.Then select Sign, and click Add Signature.Click Fill & Sign in the toolbar on the right.Open the PDF document in Adobe Acrobat Reader.But first, make sure you have the most recent version installed to enjoy the latest features. Prefer using other platforms? We’ve got you covered! Learn how to eSign a PDF in Adobe Reader Add multiple signers if you need to send the PDF to more than one. Now that you know how to sign a PDF yourself, do you want to send it out for signatures? You can request signatures by simply adding the email address of the signer. Tap the blue “Sign” button at the bottom which will then ask you “Who needs to sign?” Choose the “Sign Yourself” option. You can even import documents from one of our many app integrations such as Evernote, Google Drive, Box, Dropbox, or OneDrive. Now, import the PDF file from where it is saved on your device to add the signature. Why should you login? Because we can save your custom settings and keep your signature embedded and secure. If you don’t have an account yet, sign up for free. If you are using our mobile app for the first time, you can download it from the app store.Įnter your email address and password to log in. Take a look at our detailed guide below: Step 1: Log into Signeasy Draw, type, or upload an image of your signature.Click Signature, and then drag and drop the signature box.Click on Sign and choose "Sign Yourself".Open the PDF file saved on your device and import. There’s already so much to do in RuneScape, but we update the game very frequently which keeps players’ interests and gives them plenty of options for how they want to play. Tuckwell: Accessibility and high quality game play is key and at Jagex we always keep that in mind when considering options for RuneScape and our other games, this ensures that we always remain competitive and is one of the reasons that players are drawn to our games. Hello everyone, I have recently decided to stream my progress on RSC Preservation as a means to motivate myself to play more and interact with the community. ![]() How/why does RuneScape continue to thrive? What draws players to RuneScape’s world, and what keeps them there? In addition, turn-based RPGs on Facebook seem more popular than ever. GR: The free-to-play MMO market has exploded over the last few years. ![]() More than a third of our players are aged 19 and over.Ībove: “RuneScape is the second largest MMO in the US…” says Tuckwell We have an extremely diverse demographic of players, the majority of our players are aged 13 – 18 with only 8% of our players aged under 13. RuneScape is also available in French, German and Brazilian-Portuguese. Instead of this, by paying for membership on the current version of the game (RuneScape 3), players will automatically receive access to RuneScape Classic as. There is no way to directly pay for membership in RuneScape Classic. RuneScape is the second largest MMO in the US and is also highly popular in other parts of the English-speaking world. The pay-to-play version of the game was first announced on 4 October 2001, and officially released on 27 February 2002. Tuckwell: RuneScape has millions of players globally but the majority of our players are based in the United States. ![]() GR: Are there any regions where RuneScape is more popular? Is there anything interesting you can tell us about the demographics of its players? ![]() You can use Finale music notation software on most any modern personal computer that meets the specifications listed below. The following lists the computer and hardware requirements for using Finale 2014. No matter how you want to share, Finale has you covered. Finale files that can be shared with users of the entire Finale family of products: Finale PrintMusic, Finale SongWriter, and the free Finale NotePadĪnd of course, you can always print your music and pass it out.PDF files from entire pieces to small excerpts.When youre ready to share your music with the world, Finale provides you with more options than any other notation software. How you choose to communicate is up to you. Quickly produce flashcards, puzzles, and exercises for classes in general music, theory and ear training, composition, arranging, and jazz improvisation. Assign the piece to your students, assess their performance, and then celebrate their success.įinale provides hundreds of customizable music education worksheets. Instantly save your Finale creations as SmartMusic solo or ensemble accompaniments. SmartMusic integration, a Finale exclusive Only Finale provides you with the flexibility to create anything you can imagine. However, should you choose to customize ANY aspect of your music, Finale lets you do that too. Simply choose a Document Style from Finales Setup Wizard, and Finale handles all the details. From music fonts to chord symbols, from time signatures to music spacing, from lyrics to percussion notation, how your music appears is up to you. Personalize your music to fit your style. Whether youre creating a lead sheet for a folk song or pushing the boundaries of music notation, Finale lets you achieve anything you can imagine. Whether you enter your notes with a MIDI keyboard or a mouse, Finales exclusive Human Playback ensures that your music plays back with all the feeling, phrasing, and nuance of a live performance.įinale makes it easy to achieve printed perfection. Your music sounds better with Human Playback ![]() Finale also includes sounds from Tapspace Virtual Drumline. With Finale you get a selection of world-class instrument sounds from Garritans Personal Orchestra, Jazz & Big Band, Concert & Marching Band, and World Instruments libraries. You can even capture a brass or woodwind performance with Finales MicNotator. Scan sheet music or import a wide variety of file types, such as MIDI and MusicXML. Play in your notes with a MIDI keyboard, mouse, or computer keyboard. Finale even offers creative and editorial input, from automatically harmonizing your melody to indicating when an instrument is out of range. In an instant you can move measures, change keys, or perform dozens of edits to capture, arrange, perfect, and print anything you can imagine. Capture ideas as fast as you can with no obstacles to slow you down. When inspiration strikes, Finale takes care of the details so you can focus on your music. Finale 2014 is the forward-looking choice with new innovations, making Finales trademark freedom and flexibility easy for everyone to use.īegin creating your musical legacy today. Whether youre creating a simple lead sheet, making worksheets for your students, or composing your magnum opus, Finale helps you easily capture your musical ideas, produce beautiful notation, and quickly share the results.įor 25 years, Finales unique ability to create anything on the printed page has remained undisputed while other music notation software has come and gone. To notate music is to look forward to the performance, and beyond. Recorders, Classroom & Plastic Instruments.Signal Processors, Converters & Preamps.Using the submenu of the Measurement Units command, you can select the measurement unit you want PrintMusic to understand-and display-in all of its dialog boxes. In a number of PrintMusic dialog boxes, you’re asked to specify a measurement: the margins of each page, for example. These updates can be downloaded to registered users. Choose this menu item to view the latest updated version of PrintMusic from MakeMusic via the Internet. See Check for Updates Automatically? dialog box. If an update is available, you will be notified the next time you launch PrintMusic. These updates include incremental feature additions and solutions to defects. Choose this menu item to have PrintMusic regularly check for maintenance updates. Choose this command to display the Preferences dialog box, where you control many program-wide options. This dialog box displays the user's name and serial number along with the current software version number and ways to contact Customer Support. What it doesĪs in many computer programs, PrintMusic’s application menu contains program-wide preferences. The Finale PrintMusic 2014 menu is the first of PrintMusic’s unchanging menus. You are here: Finale PrintMusic 2014 menu How to get there ![]() These include miniguns, AK-47s, RPGs, sniper rifles, katanas, and many other amazing weapons. Amazing weapons for a better fighting experience.Various skins including the wonderful skins of the famous Minecraft game.You have the opportunity to do anything in this world. The gaming megalopolis is huge and has skyscrapers.These include Pixel Hunter, Street Race, Infection Zombie, Free PVP Fight, Team Deathmatch, and Tank Battle Modes. Here are some awesome Block City Wars features: Amazing featuresĪs mentioned earlier, this game has a lot of fun. This is without a doubt an interesting game to play on your mobile device. There are a variety of great cars to drive and escape the police. Can you complete this operation? You have complete control over the goals and determine how they should be. When you download the game you will become a mafia boss who will give you impossible tasks. Download Block City Wars to experience all the excitement it has to offer. There are many types of armor in this game. It has super rifles, destroyers, and plasma cannons. This is sure to give you guns, rage, and coins.īlock City Wars is one of those games where you will experience the most difficult arena of 3D warfare. Almost everyone in this game is a hunter, so you have to escape from them. It is worth noting that this mission is all about cops and mafia, cops and thugs, and ponies. It includes interesting missions that you can find and complete while playing. There are many amazing things about this game. Anything is possible on these roads, so extra care must be taken. There are virtually no laws or regulations on the roads of block city wars. This is a game that requires everyone to shoot all their keen senses to play. You will see all this with every move of the game. Download this game to enter the modern realm full of sniper duos, funny bandits, and fast cars. ![]() The veni, vidi, vici tattoo can be placed on almost any part of the body depending on how it is set up. ![]() It’s a bold style of writing which is fitting for a phrase that was coined after such a bold victory. Because of the connection with the Roman Emperor, Julius Caesar, it’s no surprise that people would want this tattoo inked in a style that is commemorative of Roman times. You might also see these words written in Roman numeral style writing. Therefore, it makes sense to put the veni, vidi, vici tattoo between this wreath. It is generally likened to Roman times as many Roman Emperors wore these wreaths. The laurel wreath is made from interlocking branches from the bay laurel. Many times, you’ll see the veni, vidi, vici tattoo inked between a laurel wreath. You tend to see many different styles and ways this tattoo can be inked on the body. It has also been used in music by the likes of Jay-Z on his song “Encore” and the Swedish group The Hives when they titled their album Veni Vidi Vicious. In the case King Jan III of Poland, he stated, “We came, We saw, God conquered” after the Battle of Vienna. It’s been used in military context for many years and many will add a spin to the phrase. The popularity of this phrase has increased over the years. Suetonius, a Roman historian of the time, stated that as Caesar returned home, he was led by the light of a torches and a tablet that had the phrase veni, vidi, vici inscribed. This was a great victory by Julius Caesar and was so quick and thorough that he decided to commemorate it with the phrase we’ve been speaking of, veni, vidi, vici. Initially, the attack took Caesar by surprise but once the veteran army got their bearings, they pushed the Pontic army back down the hill and ended the battle quickly. It did catch Caesar’s forces off guard, but the surprising part was that Pharnaces would give up high ground and attack going uphill. Both armies set up camps on hilltops but in a surprise move, the Pontic army unexpectedly attacked. ![]() In today’s world, this location is in northern Turkey in the Tokat province. The city of Zile ended up being location of the fight. Most wouldn’t walk into a situation like this but Pharnaces had to be dealt with for his actions toward the Roman people. He did have a strong core however, so he was confident. In contrast to Caesar’s army, Pharnaces forces consisted of many tribal forces and levied infantry. He had somewhere around 11,000 soldiers ready to fight while Pharnaces had an army of around 20,000. However, of the forces he had with him, they were veterans and battle hardened. Caesar refused this offer of peace and continued to advance.Ĭaesar would be outnumbered in this fight. Once Pharnaces heard that Caesar was on his way to deal with the issue, he sent out a peace seeking envoy. Pharnaces then committed war crimes and atrocities against the Roman prisoners and any Romans he found in the area. Pharnaces had just defeated Caesar’s army at the Battle of Nicopolis. This was after the defeat of the Ptolemaic army in the Battle of the Nile. The story behind the Battle of Zela starts when Caesar left Egypt and was travelling through Syria. In other words, veni, vidi, vici means he came in, took what he wanted and left. He had just accomplished dominating victory in the Battle of Zela over Pharnaces II of Pontus. He used this phrase when writing a letter to the Roman Senate at around 47 BC and after battle. When Caesar used this phrase, the braggadocios figure of speech shocked and impressed many writers of the time. Veni, vidi, vici is the famous Latin phrase touted by Julius Caesar who was emperor over Rome from 49 BC to 44 BC. We’ll take a look into the origins of this phrase and come to a better understanding of why people like to get the veni, vidi, vici tattoo. A statement that holds this much meaning must have an interesting story behind it. The veni, vidi, vici tattoo speaks volumes about the person wearing this ink. It’s no surprise that you’ll see this phrase tattooed on men and women alike. It translates to, I came, I saw, I conquered. Veni, vidi, vici is a Latin phrase attributed to Julius Caesar. Your abilities are on L2/R2 (LT/RT) while your Quintessence (think special pick-up ability) is on R1. While it was on its way out anyway, my Xbox 360 Wired Gamepad finally decided to die when playing this game and would refuse to stay connected. You have a basic attack with Square (X on an Xbox gamepad), jump is on X (A on Xbox) and dash is Circle (B). The base gameplay of Skul: The Hero Slayer isn’t too special on a very cursory look. This story has shades of something like Disgaea but doesn’t go quite “You are the prince of the underworld.” It’s a cute enough little story though and keeps you somewhat invested in trying to complete the game. You are found by a demonic witch and then given the mission to ultimately rescue the Demon King. You play as Skul a lowely skeleton soldier that manages to survive the assault. The Demon King is captured and the underworld is in disarray due to humanity and the main hero coming down and wrecking everything. The Story Skul The Hero Slayer/Marc Morrison/SouthPAW Games & NEOWIZįor what it is, the story is actually kind of interesting. It’s pretty old-hat roguelike gameplay at this point. Once you are killed, you start off at zero and try the run again. You do runs of the game stages where you start off with nothing and try to become more and more powerful during a run. Skul: The Hero Slayer is a 2D Roguelike game in the vein of Dead Cells, Risk of Rain, Rogue Legacy and so on. ![]() Skul The Hero Slayer: Not Enough To Keep Me Coming Back Skul is not a bad game at all, but the bar is set so high now with the Roguelike genre that even being decent isn’t quite good enough. ![]() However, the more I played of it, the more frustrated I became. When I saw the trailer for Skul The Hero Slayer for the first time, I thought to myself, “Yep that’s totally for me.” I tend to like roguelikes, and I liked the idea that you are basically a bad guy trying to take down the hero that supposedly won. ![]() The diversification of BCR is formed by 83 frequent somatic changes of germline DNA, which generates unique sequences and 84 facilitates our body defense against. The majority of the substitutions that still allowed binding were 3′ to the central C residue indicating that the two sides of the palindromic recognition sequence are not equivalent. B cell receptor assembly and repertoire diversification 81 BCRs are composed of membrane-bound immunoglobulins that can identify and bind 82 to specific antigens (Hoehn et al., 2016). ![]() The most common variation was the C to A at position +2. In addition, our data indicate that GCN4 can bind with lower affinity to sequences that differ from the optimal sequence in one or even two positions. Using a total of 200 ng of purified protein and four cycles of binding and subsequent amplification, we identified the TGA(C/G)TCA sequence as the binding consensus of GCN4, which is consistent with the previously reported recognition sequence. We used this method to define the sequence requirement of the binding domain of the yeast transcriptional activator GCN4. The tumor suppressor protein p53 acts as a transcription factor, binding sequence-specifically to defined DNA sites, thereby activating the expression of genes leading to diverse cellular outcomes. The method employs polymerase chain reaction (PCR) amplification of sequences selected from a mixture of random oligonucleotides by the gel mobility-shift assay. Nearly all of biology depends on interactions between molecules: proteins with small molecules, proteins with other proteins, nucleic acids with small. The following processes that participate in the generation of antibody diversity were summarizedallelic, combinational, and junctional diversity, pairing of IgH and IgL, and receptor editingwhich all together produce the primary antigen repertoire (pre-antigen stimulation). We developed a simple and accurate method to define the sequence recognition properties of DNA-binding proteins. |
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